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Genomic selection for accelerated heartwood formation in Pedunculate oak (Quercus robur L.) using whole-genome sequencing

Lobo, A.; Doonan, J. M.; Hansen, J. K.; Kosawang, C.; Xu, J.; Olofsson, J.; Kjaer, E. D.

2025-07-25 genetics
10.1101/2025.07.22.666121 bioRxiv
Show abstract

Heartwood traits in trees are critical for timber quality but are notoriously difficult to phenotype due to their late expression and the need for destructive sampling. In this proof-of-concept study, we demonstrate that combining genome-wide association studies (GWAS) with Bayesian genomic prediction models provides an effective strategy to overcome these challenges. By using GWAS to preselect trait associated SNPs and integrating them into predictive models, we substantially improve the accuracy of genomic predictions for heartwood related traits in oaks. Our approach allows for reliable selection of superior genotypes at the seedling stage, long before heartwood traits can be directly measured, thus enabling early and cost-effective breeding decisions. We also identify the number of rings in sapwood as a genetically controlled, easily measured proxy trait that enhances selection strategies for heartwood content. Together, these findings provide a scalable framework for integrating genomics into operational tree breeding programs and demonstrate how combining GWAS and genomic prediction can accelerate the improvement of complex wood traits in long lived forest tree species.

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