Synthetic autotrophic yeast enables high itaconic acid production from CO2 via integrated pathway and process design
Ata, O.; Lutz, L.; Baumschabl, M.; Mattanovich, D.
Show abstract
Single carbon (C1) substrates are gaining importance as future feedstocks for the production of bio-based chemicals. Carbon dioxide, a major greenhouse gas, offers a promising alternative to the traditional feedstocks to shift towards C1-based, sustainable processes. Here, we present a synthetic autotrophic Komagataella phaffii (Pichia pastoris) that is able to produce itaconic acid by the direct conversion of CO2, achieving final titers of approximately 12 g L-1 in bioreactor cultivations. We show that a combined approach that integrates balancing the flux between the Calvin-Benson-Bassham (CBB) cycle and itaconic acid metabolism with process design was essential to enhance the production. Our study demonstrates the potential of K. phaffii as a microbial platform using CO2 as the direct carbon source, aligning with the future goals of establishing sustainable bioprocesses.
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