Two de novo transcriptome assemblies and functional annotations from juvenile cuttlefish (Sepia officinalis) under various metal and pCO2 exposure conditions
Sol Dourdin, T.; Minet, A.; Pante, E.; Lacoue-Labarthe, T.
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AO_SCPLOWBSTRACTC_SCPLOWThe cuttlefish Sepia officinalis is a precious model in behavioural and neurobiology studies. It is currently facing combined environmental changes related to the anthropogenic global change. However, genomic resources available to support investigations tackling this issue are still scarce. Therefore, we present two annotated de novo transcriptome assemblies from recently hatched (whole body) and one-month-old (head) Sepia officinalis juveniles. Both assemblies rely on an important read depth validated by a pseudo-rarefaction analysis, and gathered several individuals from various metal and pCO2 exposure conditions. After redundancy reduction, assemblies from newly hatched and one-month-old individuals comprised 230,672 and 370,613 transcripts with 35,590 and 44,233 putative ORFs, respectively, and an annotation rate arounf 70%. Assemblies were compared to each other, revealing age-specific transcriptomic landscapes. These two assemblies constitute highly valuable genomic resources complementing reference genome assembly and facilitating the investigation of transcriptomic endpoints in environmental studies considering coleoid cephalopods.
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