Exploring the diagnostic and prognostic utility of resting state functional MRI connectivity in acute/sub-acute TBI

Traumatic brain injury (TBI) is a leading cause of disability worldwide. Yet, our understanding of the mechanisms of this condition is limited, especially in the acute setting. Here, we investigated the relationship between functional connectivity and common clinical assessments, like the Glasgow Coma Scale (GCS) at admission and modified Rankin scale at 3-months (mRS) to determine if functional connectivity can provide a broader representation of the brains networks than these standard tests. We performed a retrospective analysis of resting state functional MRI and clinical data in 58 patients (41.28 {+/-} 18.63) scanned acutely/subacutely ([&le;] 31 days). Then, for a secondary analysis, we included 50 more patients who presented after either a first or a repeat incident and were scanned either acutely/subacutely or chronically (<2 yrs) (all together 108 patients, 46.4 {+/-} 20.1yrs). Using a 268-node functional atlas, we derived 35,778 unique edges, based on which we calculated the mean functional connectivity of 10 resting state networks and used those to establish a link to TBI severity and functional outcome. Our analysis showed that when dividing sub/acute patients (n=58) based on GCS severity, only the Subcortical network showed a significant discrimination between mild and moderate-severe GCS at admission (P<0.001), with hyperconnectivity noted in mild patients, and hypoconnectivity - in moderate-severe GCS patients. This difference appeared to be mainly driven by the thalami (Right, P=0.002; Left P<0.001). Similar results were observed when investigating GCS subscores at admission (Eyes, Motor, Verbal, all P<0.001). Further, when evaluating mRS outcomes at 3-months against functional connectivity, differences were noted within the Motor, Cerebellum and Medial-Frontal networks, though none survived multiple comparisons. Importantly, we found the DMN and mRS to be correlated but with a limited relationship (r2= 0.18). Lastly, we performed a post-hoc analysis (n=108) to investigate if the hyperconnectivity in the Subcortical network of sub/acute mild GCS patients remained irrespective of acuity of scanning (i.e. acute/ chronic) or frequency of TBI (i.e. first/ repeat). Our analysis showed that GCS severity appeared to be the main driver of functional connectivity within the Subcortical network, whereas acuity of scanning, alongside GCS severity contributed to the results of chronically scanned patients. While GCS and 3-month mRS scores offer some meaningful insights, their limited capture of the neural representation underscores the need to investigate whether other early clinical assessments correlate more robustly with early resting state networks or whether such networks themselves could predict future outcomes.