Backbone Assignment of a 28.5 kDa Class A Extended Spectrum β-Lactamase by High-Field, Carbon-Detected Solid-State NMR
Williams, C. G.; Wang, S.; Thome, A. F.; Warmuth, O. A.; Sakhrani, V.; Rienstra, C. M.; Mueller, L. J.
Show abstract
13C and 15N backbone chemical shift assignments are reported for the 28.5 kDa protein Toho-1 {beta}-lactamase, a Class A extended spectrum {beta}-lactamase. A very high level of assignment completeness (97% of the backbone) is enabled by the combined sensitivity and resolution gains of ultrahigh-field NMR spectroscopy (1.1 GHz), improved probe technology, and optimized pulse sequences. The assigned chemical shifts agree well with our previous solution-state NMR assignments, indicating that the secondary structure is conserved in the solid state. These assignments provide a foundation for future investigations of sidechain chemical shifts and catalytic mechanism.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Using solid-state NMR to understand the structure of plant cellulose 95%
- Multiplexed analysis of multicomponent biomolecular condensates without any tag 94%
- Proton-Detected Solid-State NMR for Deciphering Structural Polymorphism and Dynamic Heterogeneity of Cellular Carbohydrates in Pathogenic Fungi 93%
Similar papers in this journal
- An Inherent Structural Difference Between Serine and Threonine Phosphorylation: Phosphothreonine Prefers an Ordered, Compact, Cyclic Conformation 92%
- Probing the KRas Switch II Groove by Fluorine NMR Spectroscopy 92%
- Chemoproteomic study uncovers HemK2/KMT9 as a new target for NTMT1 bisubstrate inhibitors 91%
Similar papers in this journal
- Structural dynamics of cytochrome P450 3A4 in the presence of substrates and cytochrome P450 reductase 91%
- Double mutant of chymotrypsin inhibitor 2 stabilized through increased conformational entropy 90%
- Metabolic Processing of Selenium-based Bioisostere of meso-diaminopimelic Acid in Live Bacteria 90%
Similar papers in this journal
- High-Throughput Measurement and Machine Learning-Based Prediction of Collision Cross Sections for Drugs and Drug Metabolites 91%
- Tracking the Metabolic Fate of Exogenous Arachidonic Acid in Ferroptosis Using Dual-Isotope Labeling Lipidomics 89%
- ModiFinder: Tandem Mass Spectral Alignment Enables Structural Modification Site Localization 89%
Similar papers in this journal
- Rapid High-Resolution Analysis of Polysaccharide-Lignin Interactions via Proton-Detected Solid-State NMR with Application to Eucalyptus and Spruce 94%
- Microsecond resolved infrared spectroscopy on non-repetitive protein reactions by applying caged-compounds and quantum cascade laser frequency combs 91%
- Development of a Platform for High-Resolution Ion Mobility Separations Coupled with Messenger Tagging Infrared Spectroscopy for High-Precision Structural Characterizations 90%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.