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Control with Practical Guarantees of Stationary Variance in Stochastic Chemical Reaction Networks

M. Zand, A.; Gupta, A.; Khammash, M.

2025-05-12 synthetic biology
10.1101/2025.05.12.653489 bioRxiv
Show abstract

Biomolecular integral feedback controllers offer precise regulation of molecular species copy numbers, making them valuable for synthetic biology applications. Antithetic integral feedback controllers, in particular, can be effective in low-copy-number regimes with stochastic dynamics. In this work, we introduce a modified variant of this controller, called the antithetic dual-rein integral feedback motif, and analyze its performance from a stochastic perspective in the presence of intrinsic dynamic randomness. We demonstrate that our controller enables first-moment control while maintaining a tractable steady-state variance bound under specific parametric regimes. Notably, this variance bound is tunable, as it depends solely on the controller parameters. We derive these results using stochastic model-order reduction and validate them through numerical simulations. Our findings provide new insights into achieving both precise regulation and noise suppression in stochastic genetic circuits.

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