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First chromosome scale genome of Acrocomia aculeata

Scaketti, M.; Garcia, C. B.; Araujo, J. V. d. S. R.; de Carvalho, I. A. S.; Marroquin, J. A. M.; Riano-Pachon, D. M.; Neto, D. D.; Labate, C. A.; Vicentini, R.; Pinheiro, J. B.; Vianna, S.; Colombo, C.; Zucchi, M. I.

2025-05-15 genomics
10.1101/2025.05.12.653347 bioRxiv
Show abstract

The Arecaceae family comprises economically, and ecologically significant palm species widely distributed across tropical and subtropical regions. Among them, Acrocomia aculeata, commonly known as Macauba, has gained attention due to its high oil yield, environmental adaptability, and potential applications in sustainable agriculture and bioenergy production. This study presents the first chromosome scale genome assembly of A. aculeata and the first reference genome of the genus, using Oxford Nanopore Technologies sequencing, PacBio HiFi and Hi-C proximity ligation data. The genome covers 1.94 Gbp in the 15 pseudochromosomes, with N50 of 143.43 Mbp, achieving a base quality (QV) of 76.4 and a k-mer completeness of 90.58% of PacBio HiFi data, with k=21. Interspersed repetitive regions represent 77.36% of the total genome, with long terminal repeat (LTRs) accounting for 54.98%. A total of 28.215 protein-coding genes were predicted. The genome was validated with BUSCO, achieving a completeness of 98.9% for predicted proteins. The first chromosome scale genome of Macauba provide new insights and breakthroughs for studies of conservation genomics and plant breeding programs.

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