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Phase-field Approach to Cellular Blebbing

Ji, K.; Levine, H.; Karma, A.

2025-03-17 biophysics
10.1101/2025.03.14.643157 bioRxiv
Show abstract

Bulges in the plasma membrane of cells known as blebs can form spontaneously in a wide range of biological processes but what controls their shape and stability remains incompletely understood. To address this, we introduce a dual phase-field model with coupled order parameters representing the cell cortex and plasma membrane that can quantitatively model blebbing in three dimensions. Simulations and analysis of the model reveal that, depending on whether blebbing occurs by detachment of the plasma membrane or rupture of the actin cortex, blebs can form discontinuously through a saddle node bifurcation or continuously with increasing cortical tension. The model predictions are in good quantitative agreement with existing experimental data for laser-induced cortex rupture.

Published in Physical Review E (predicted rank #3) · training set

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