An Experimental and Multiphysics Simulations Study of Clostridium carboxidivorans sp. 624 for Acid and Alcohol Production in CO2/H2
Tiong, Y. W.; Yong, A.; Nalaparaju, A.; Nguyen, H.-H.; Koh, L. Q.; Wong, R. J.; Lim, R. T. Y.; Ow, D. S.-W.; Zhang, J.; Nguyen, V. B.; Wong, F. T.; Lim, Y. H.
Show abstract
Biotechnological advances in CO2 utilization through gas fermentation offer a sustainable alternative to energy-intensive chemical processes. This study investigates and optimizes fermentation dynamics of Clostridium carboxidivorans sp. 624 for enhanced production of C2-C6 acids via combined experimental and computational methods. A systematic experimental evaluation of temperature and medium conditions, along with time-course analysis elucidates metabolic pathway regulation. Experimental optimisation, complemented by modelling, identified gas-liquid volumetric ratios VL/VG = 4 as optimal for maximizing longer-chain acids production, attributed to enhanced gas solubility and substrate bioavailability. Additionally, a hybrid model combining dynamic-kinetic model and computational fluid dynamics (CFD) successfully predict product formation. By capturing spatial inhomogeneities in gas-liquid interactions, the model also provides critical insights for optimizing fermentation performance and establishes a framework for improving process design parameters for CO2/H2 fermentation, paving the way for scalable and efficient CO2-based bioprocesses.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- An open-source multiple-bioreactor system for replicable gas-fermentation experiments: Nitrate feed results in stochastic inhibition events, but improves ethanol production of Clostridium ljungdahlii with CO2 and H2 97%
- Cross-talk between engineered Clostridium acetobutylicum and Clostridium ljungdahlii in syntrophic cocultures enhances isopropanol and butanol production 96%
- Isolation, identification and selection of bacteria with the proof-of-concept for bioaugmentation of whitewater from woodfree paper mills 94%
Similar papers in this journal
- Lactate production from lactose-rich wastewater: A comparative study on reactor configurations to maximize conversion rates and efficiencies 96%
- Exclusive D-lactate-isomer production during a reactor-microbiome conversion of lactose-rich waste by controlling pH and temperature 96%
- Modeling Versatile and Dynamic Anaerobic Metabolism for PAOs/GAOs Competition Using Agent-based Model and Verification via Single Cell Raman Micro-spectroscopy 96%
Similar papers in this journal
- Volatile aroma compound production is affected by growth rate in S. cerevisiae 95%
- Harnessing Escherichia coli for bio-based production of formate under pressurized H2 and CO2 gases. 94%
- Quantitative physiology of non-energy-limited retentostat cultures of Saccharomyces cerevisiae at near-zero specific growth rates 94%
Similar papers in this journal
- Resource Recovery from Wastewater By Directing Microbial Metabolism Toward Production of Value-added Biochemicals 94%
- Marine Bacteria Associated with the Green Seaweed Ulva sp. for the Production of Polyhydroxyalkanoates 94%
- Pseudomonas putida JM37 as a novel bacterial chassis for ethylene glycol upcycling 94%
Similar papers in this journal
- Low cost and sustainable hyaluronic acid production in a manufacturing platform based on Bacillus subtilis 3NA strain 96%
- Operational and biochemical aspects of co-digestion (co-AD) from sugarcane vinasse, filter cake and deacetylation liquor 95%
- The feedstock microbiome selectively steers process stability during the anaerobic digestion of waste activated sludge 94%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.