Optimizing Transfection Efficiency in Primary Human Splenic Fibroblast Cells
Kaur, A.; Assogba, V. I.; Assogba, B. D.
Show abstract
Transfection, a fundamental molecular biology technique, is crucial in introducing foreign DNA into cells. Despite its pivotal importance, mastering this technique remains a formidable challenge. In this study, we employed the calcium phosphate reagent and conducted a systematic exploration through time-dependent experiments to assess its impact on human primary splenic fibroblast cells (HSFC). The results precisely delineate optimal time frames for achieving enhanced transfection efficiency, offering conclusive evidence for the efficacy of calcium phosphate in transient transfection within the cells. The data significantly advances our comprehension of HSFC reactions, contributing to refining transfection methodologies with broader implications for biomedical applications.
Matching journals
The top 10 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Characterization of CRISPR/Cas9 RANKL knockout mesenchymal stem cell clones based on single-cell printing technology and emulsion coupling assay as a low-cellularity workflow for single-cell cloning 94%
- RecombiCraft Library construction: A novel method for DNA Library cloning and expansion using non-enzymatic single-step DNA recombination and liquid culture 93%
- Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire 93%
Similar papers in this journal
- Osteogenic differentiation potential and marker gene expression of different porcine bone marrow mesenchymal stem cell subpopulations selected in different basal media 91%
- A New Software Tool for Computer Assisted In Vivo High-Content Analysis of Transplanted Fluorescent Cells in intact Zebrafish Larvae 91%
- Nanomorphological and mechanical reconstruction of mesenchymal stem cells during early apoptosis detected by atomic force microscopy 90%
Similar papers in this journal
- Development of a Serum-Free Producer Cell Line Generation Process for Scalable and Efficient rAAV Production for gene therapy applications 95%
- A bead-based method for the removal of the amino acid lysine from cell-free transcription-translation systems 92%
- Rapid, high-yield production of full-length SARS-CoV-2 spike ectodomain by transient gene expression in CHO cells 92%
Similar papers in this journal
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.