Polarization light-sheet microscopy and tomography (PLμTo) for flow-based imaging of 3D microcarrier mesenchymal stem cell culture
Benavides, O. R.; White, B. P.; Kaunas, R.; Gregory, C. A.; Walsh, A. R.
Show abstract
Light-sheet microscopy is an increasingly popular imaging tool for studying biological processes, however, it generally requires the use of exogenous fluorescent contrast agents which can be toxic to live cells. Light-sheet tomography is the label-free analog of light-sheet microscopy, utilizing elastic scattering contrast to visualize the structure of the biological sample. Here, we present a multi-modal polarization light-sheet microscopy and tomography (PL{micro}To) system for on-line, non-destructive, and non-invasive volumetric imaging of human mesenchymal stem cells, also referred to as mesenchymal stromal cells, attached to spherical gelatin methacryloyl microcarriers for image analysis-based quantitative monitoring of adherent stem cell culture expansion. The PL{micro}To system, which is built on an inverted selective plane illumination configuration, is compatible with standard horizontal sample mounting and microfluidics for multimodal volumetric data acquisition. This work is the first demonstration of scattering-based volume optical imaging flow cytometry.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Multicolor fluorescence microscopy for surgical guidance using a chip-scale imager with a low-NA fiber optic plate and a multi-bandpass interference filter 95%
- Versatile, do-it-yourself, low-cost spinning disk confocal microscope 95%
- An integrated multi-wavelength SCATTIRSTORM microscope combining TIRFM and IRM modalities for imaging cellulases and other processive enzymes 95%
Similar papers in this journal
- An Augmented Reality Visor for Intraoperative Visualization, Guidance and Temperature Monitoring using Fluorescence 94%
- A waveguide imaging platform for live-cell TIRF imaging of neurons over large fields of view 94%
- Single-shot phase contrast microscopy using polarisation-resolved differential phase contrast 93%
Similar papers in this journal
Similar papers in this journal
- Intracellular label-free detection of mesenchymal stem cell metabolism within a perivascular niche-on-a-chip 93%
- Gut-on-Chip Methodology Based on 3D-Printed Molds: A Cost-Effective and Accessible Approach 93%
- Injection molded open microfluidic well plate inserts for user-friendly coculture and microscopy 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.