Detection and quantification of hepatitis A virus titers from wastewater in South Africa and comparison with clinical data from the National Surveillance Database

2024-12-21 infectious diseases Title + abstract only

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Wastewater surveillance is useful for monitoring the prevalence of hepatitis A virus (HAV). We developed and optimized HAV detection and quantification methods for wastewater samples, and applied them to samples collected through a national wastewater surveillance program. Previously identified 5-untranslated region-targeting primers and probes were used to develop the assay. Serial dilutions of HAV-positive clinical samples were used to validate and determine limits of quantification (LOQ). Ret...

Detection and quantification of hepatitis A virus titers from wastewater in South Africa and comparison with clinical data from the National Surveillance Database

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