The sugar beet root maggot, Tetanops myopaeformis transcriptome sample statistics in relation to it experiencing susceptible and resistant reactions with sugar beet, Beta vulgaris ssp. vulgaris
Acharya, S.; Alkharouf, N. W.; Tehseen, M.; Chu, C. P.; Klink, V. P.
Show abstract
The sugar beet root maggot (SBRM), Tetanops myopaeformis (von Roder), is a devastating insect pathogen of sugar beet (SB), Beta vulgaris ssp, vulgaris (B. vulgaris), one of only two plants in the world from which significant global raw sugar is produced, $1 billion, U.S., $4.6 B, globally. Experiments reveal the SBRM larval transcriptome experiencing two different susceptible or resistant responses by sugar beet SBRM larvae were sampled at time = 0 hours post infection [hpi]), prior to being introduced to B. vulgaris and after infection on F1016 and F1024 (resistant), and F1010 and L19 (susceptible) for 24, 48, and 72 hpi when the larvae were removed for transcriptomic analysis. The transcriptomic analyses included determining the number of reads per sample, mapping the transcripts to the recently sequenced SBRM TmSBRM_v1.0 draft genome, identifying genes that relate to the resistant and susceptible responses. Moreover, the RNA-seq experiments provide data for generating differential expression analyses between the various sample types, thus, yielding an understanding SBRM biology, the development of new control strategies for this pathogen, relationship to model genetic organisms like Drosophila melanogaster, relationship to pathogenic non-model organisms, and aid in agronomic improvement of sugar beet for stakeholders.
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