Molecular Characterization And Promoter Element Analysis Of Growth Regulator Bragrf5 In Brassica Campestris L. Ssp. Chinensis
Wang, X.-Y.; Zhang, J.; Qi, X.-H.; Li, G.-Z.; Lan, C.-Y.; Li, Q.-Z.
Show abstract
The transcriptional regulation of eukaryotic gene expression is a critical aspect, with transcription factors playing a key role by combining with cis-acting elements in the promoter regions. Growth regulating factors (GRFs) are plant-specific transcriptional factors. The Arabidopsis thaliana growth-regulating factor gene (AtGRF5), when ectopically expressed in crops such as Beta vulgaris (sugar beet) and Brassica napus (canola), has an effect of accelerating shoot organogenesis and enhancing the efficiency of genetic transformation. However, an efficient plant regeneration and genetic transformation system in Brassica campestris L. ssp. chinensis, a major vegetable crop in China, remains to be established. In this work, we identified and characterized the AtGRF5 ortholog in B. campestris, named as BraGRF5, from BRAD database. Molecular analysis of BraGRF5 gene and exploration of its protein interaction network were further conducted. Therefore, the promoter region of BraGRF5 was thoroughly examined to determine cis-acting elements of various kinds and quantities. It is expected to provide a foundation for regulation of BraGRF5, and a informative reference for the improvement of B. campestris transformation efficiency and molecular breeding.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Transcriptome sequencing and screening of genes related to sex determination of Trichosanthes kirilowii Maxim. 98%
- Genome-wide analysis of chalcone synthase (CHS) family from eggplant (Solanum melongena L.) in flavonoid biosynthetic pathway and expression pattern in response to heat stress 97%
- Identification of heat responsive genes in pea stipules and anthers through transcriptional profiling 96%
Similar papers in this journal
- Identification of novel candidate genes associated with the symbiotic compatibility of soybean with rhizobia under natural conditions 96%
- Phenotypic and transcriptomic analysis reveals early stress responses in transgenic rice expressing Arabidopsis DREB1a 95%
- Membranous interacting partners of phage-type plastid RNA polymerase have limited impact on plastid gene expression during chloroplast development 95%
Similar papers in this journal
- Molecular characterization revealed the role of thaumatin-like proteins in stress response in bread wheat 97%
- Genome-wide identification, expression and bioinformatic analyses of GRAS transcription factor genes in rice 96%
- Isoprenoid biosynthesis regulation in poplars by methylerythritol phosphate and mevalonic acid pathways 96%
Similar papers in this journal
- In silico characterization of MTP1 gene associated with Zn homeostasis across different dicot plant species 97%
- A Transcriptomic Atlas of Macauba Palm Reveals Organ-Specific Gene Expression and Stress-Related Pathways 95%
- In silico analysis of SNPs in human phosphofructokinase, Muscle (PFKM) gene: An apparent therapeutic target of aerobic glycolysis and cancer 91%
Similar papers in this journal
- Genome-wide identification, characterization and expression analysis of CsC3H gene family in cucumber (Cucumis sativus L) under various abiotic stresses 97%
- Modulation of the DA1 pathway in maize shows that translatability of information from Arabidopsis to crops is complex 96%
- Structure-function relationship of Gossypium hirsutum NAC transcription factor, GhNAC4 with regard to ABA and abiotic stress responses 96%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.