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CRAK-Velo: Chromatin Accessibility Kinetics integration improves RNA Velocity estimation

El Kazwini, N.; Gao, M.; Boudjelthia, I. K.; Cai, F.; Huang, Y.; Sanguinetti, G.

2024-09-17 bioinformatics
10.1101/2024.09.12.612736 bioRxiv
Show abstract

RNA velocity has recently emerged as a key tool in the analysis of single-cell transcriptomic data, yet connecting RNA velocity analyses to underlying regulatory processes has proved challenging. Here we propose CRAK-Velo, a semi-mechanistic model which integrates chromatin accessibility data in the estimation of RNA velocities. CRAK-Velo provides biologically consistent estimates of developmental flows and enables accurate cell-type deconvolution, while additionally shining light on regulatory processes at the level of interactions between genes and chromatin regions.

Published in Genome Biology (predicted rank #2) · training set

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