Back

Enhancement strategies for the microbial protein production of nitrogen-fixing hydrogen-oxidizing bacterial community

Wang, H.

2024-08-07 microbiology
10.1101/2024.08.07.607053 bioRxiv
Show abstract

The feasibility and superiority of utilizing nitrogen-fixing hydrogen-oxidizing bacteria (NF-HOB) for microbial protein (MP) production have been proposed. This study simulated air as the source of nitrogen and oxygen and enhanced production efficiency by employing key strategies, including continuous gas supply, selecting a higher hydrogen-to-oxygen ratio, designing initial community structures and domestication, and exploring appropriate hydraulic retention times (HRT) in continuous culture. The results demonstrated that sequencing batch culture achieved an MP titer of 0.98 g/L, with N2 fixation efficiency surpassing natural nodules by two orders of magnitude at 1.6 mg N2 per g dry biomass per hour. Under an HRT of 48 hours, MP volumetric productivity reached 2.297 mg/(L{middle dot}h), accompanied by a maximum biomass yield of 0.11 g CDW/g COD-H2. The high abundance of nitrogenase may provide crucial functional support, enabling the NF-HOB community to exhibit potential beyond previous understanding.

Matching journals

The top 5 journals account for 50% of the predicted probability mass.

50% of probability mass above

"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.