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Evolving a High-Performance Terminal Deoxynucleotidyl Transferase for Enzymatic DNA Synthesis

Forget, S.; Krawczyk, M.; Knight, A. M.; Ching, C.; Copeland, R.; Mahmoodi, N.; Mayo, M.; Nguyen, J.; Tan, A.; Miller, M. G.; Vroom, J.; Lutz, S.

2024-06-27 bioengineering
10.1101/2024.06.24.600205 bioRxiv
Show abstract

Enzymatic DNA synthesis, using stepwise nucleotide addition catalyzed by template-independent polymerases, promises higher efficiency, quality, and sustainability than todays industry standard phosphoramidite-based processes. We report on the directed evolution of a terminal deoxynucleotidyl transferase that uses 3-phosphate blocked dNTPs to control the polymerization reaction and demonstrates high activity for these modified substrates and improved template promiscuity and thermostability.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.