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MargheRita: an R package for LC-MS/MS SWATH metabolomics data analysis and confident metabolite identification based on a spectral library of reference standards

Mosca, E.; Ulaszewska, M.; Alavikakhki, Z.; Bellini, E. N.; Mannella, V.; Frigerio, G.; Drago, D.; Andolfo, A.

2024-06-22 bioinformatics
10.1101/2024.06.20.599545 bioRxiv
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Short Structured AbstractUntargeted metabolomics by mass spectrometry technologies generates huge numbers of metabolite signals, requiring computational analyses for post-acquisition processing and databases for metabolite identification. Web-based data processing solutions frequently include only a part of the entire workflow thus requiring the use of different platforms. The R package "margheRita" enhances fragment matching accuracy and addresses the complete workflow for metabolomic profiling in untargeted studies based on liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS), especially in the case of data-independent acquisition, where all MS/MS spectra are acquired with high quantitative accuracy. Availability and Implementationsource code and documentation are available at https://github.com/emosca-cnr/margheRita. Contactettore.mosca@itb.cnr.it, andolfo.annapaola@hsr.it

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