Unraveling the effects of polyhydroxyalkanoates accumulation in Pseudomonas extremaustralis growth and survival under different pH conditions
Brito, M. G.; Lopez, N. I.; Iustman, L. J. R.
Show abstract
Polyhydroxyalkanoates (PHAs) are intracellular polymers that enhance bacterial fitness against various environmental stressors. Pseudomonas extremaustralis 14-3b is an Antarctic bacterium capable of accumulating, short-chain-length PHAs (sclPHAs), composed of C3-C5 monomers, as well as medium-chain-length PHAs (mclPHAs) containing [≥]C6 monomers. Since pH changes are pivotal in bacterial physiology, influencing microbial growth and metabolic processes, we propose that accumulated PHA increases P. extremaustralis fitness to cope with pH changes. To test this, we analyzed the production of sclPHA and mclPHA at different pH levels and its effect on bacterial survival against pH stress. P. extremaustralis was able to grow and accumulate PHA when the culture media pH ranged from 6.0 to 9.5, showing a marked loss of viability outside this range. Additionally, based on the analysis of different PHA-deficient mutants, we found that when exposed to both acidic and alkaline conditions, sclPHA and mclPHA conferred different protection against pH stress, with sclPHA making the main contribution. These results highlight the importance of PHA in supporting survival in pH-stressful environments.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Seed biopriming with P- and K-solubilizing <em>Enterobacter hormaechei</em> sp. improves the early vegetative growth and the P and K uptake of okra (<em>Abelmoschus esculentus</em>) seedling 96%
- Divulging diazotrophic bacterial community structure in Kuwait desert ecosystems and their N2-fixation potential 96%
- pH effect on strain-specific transcriptomes of the take-all fungus 96%
Similar papers in this journal
- Listening to bacterial Esperanto: transcriptome reprogramming in a plant beneficial rhizobacterium 96%
- Restriction of the growth and biofilm formation of ESKAPE pathogens by caprine gut-derived probiotic bacteria 95%
- Growth response and recovery of Corynebacterium glutamicum colonies on single-cell level upon defined pH stress pulses 95%
Similar papers in this journal
- Catabolism of 3-hydroxypyridine by Ensifer adhaerens HP1: a novel four-component gene encoding 3-hydroxypyridine dehydrogenase HpdA catalyzes the first step of biodegradation 96%
- Adaptive laboratory evolution restores solvent tolerance in plasmid-cured Pseudomonas putida S12; a molecular analysis 96%
- Paenibacillus sp. strain UY79, isolated from a root nodule of Arachis villosa, displays a broad spectrum of antifungal activity 95%
Similar papers in this journal
- The putative phosphate transporter PitB (PP1373) is involved in tellurite uptake in Pseudomonas putida KT2440 96%
- Molecular mechanism of sulfur chemolithotrophy in the betaproteobacterium Pusillimonas ginsengisoli 95%
- Shotgun proteomics analysis of nanoparticle-synthesising Desulfovibrio alaskensis in response to platinum and palladium 93%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.