Selective illumination on line-scanning confocal mesoscope enhances background rejection in cortex-wide mouse brain imaging
Xie, H.
Show abstract
Confocal microscope has optical sectioning that is accessible for structural and dynamic imaging in in vivo mouse brain. With the requirement of brain-wide field-of-view (FOV) in many neuroscience researches, existing confocal microscope fails to fulfill the requirement. Here, we proposed the brain-wide, high resolution line-scan confocal mesoscope (LSCM) for in vivo mouse imaging, achieving 6.6-mm-FOV, 3.2-m-resolution and video-rate acquisition. To further enhance the background rejection ability, we introduced the selective illumination method into our system. We demonstrated that the proposed technique is s able to image the neurodynamics in in vivo mouse brain. Comparing to the LSCM and wide-field mesoscope, our selective illumination line-scan confocal mesoscope improves more than 25% and 3 times background rejection ability, respectively.
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