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Naphthalimide-based, Single-Chromophore, Ratiometric Fluorescent Sensor for Tracking Intracellular pH

Das, S. K.; Kahali, S.; Kar, S.; Madhavan, N.; Datta, A.

2024-05-14 biochemistry
10.1101/2024.05.14.594096 bioRxiv
Show abstract

We report a novel, reversible, cell-permeable, pH-sensor, TRapH. TRapH afforded a pH-sensitive ratiometric emission response in the pH range [~]3-6, enabling imaging and quantification of pH in living cells. The biological-applicability of TRapH was illustrated via live-tracking of intracellular pH dynamics in living mammalian cells induced by a synthetic H+-transporter.

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