Engineering bacterial protein polymers to support human pluripotent stem cell growth and differentiation in culture.
Creigh, A.; Waller, H.; Lakey, J. H.; Chrzanowska-Lightowlers, Z. M.; Lightowlers, R. N.; Peters, D. T.
Show abstract
Induced pluripotent stem cells (iPSCs) are of significant value due to their wide ranging potential, removing the need for embryonic material. To successfully culture, expand and differentiate these cells, it is crucial to maintain a precise biological environment, including an appropriate attachment substrate. Commonly used attachment substrates include recombinant extracellular matrix (ECM) components like vitronectin, as well as animal-derived ECM mixes such as GelTrex and Matrigel. However, there is growing interest in exploring alternative approaches to support bioactivity of cells. One approach that is gaining traction is the use of the Caf1 protein of Yersinia pestis. This protein is appealing primarily due to its stability, modularity, and ease of production. In this study, we have developed novel variants of Caf1 that effectively support the growth and differentiation of iPSCs, performing at least as well as GelTrex. Our findings highlight the potential of Caf1 laminin and vitronectin mimics as viable alternatives for supporting iPSC growth and differentiation. The successful development of these Caf1 variants opens new avenues for the field, paving the way for better defined, more cost-effective and readily available attachment substrates in iPSC research and applications.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- High Throughput Morphological Screening Identifies Chemically Defined Media for Mesenchymal Stromal Cells that Enhances Proliferation and Supports Maintenance of Immunomodulatory Function 94%
- GMP-grade neural progenitor derivation and differentiation from clinical-grade human embryonic stem cells 94%
- Device encapsulated MSCs for adaptive secretome therapy to effectively target ischaemic heart injury 94%
Similar papers in this journal
- Uniform cerebral organoid culture on a pillar plate by simple and reproducible spheroid transfer from an ultralow attachment well plate 95%
- Collagen Hydrogel Tube Microbioreactors for Cell and Tissue Manufacturing 94%
- Biofabrication of an in situ hypoxia-delivery scaffold for cartilage regeneration 94%
Similar papers in this journal
- Effect of a retinoic acid analogue on BMP-driven pluripotent stem cell chondrogenesis 94%
- Small Extracellular Vesicles from Human Bone Marrow Mesenchymal Stromal Cells enhance migration and regulate gene expression of reparative mechanisms in human dermal fibroblasts 94%
- Dual SMAD inhibition and Wnt inhibition enhances the differentiation of induced pluripotent stem cells into Retinal Ganglion cells (iPSC-RGCs) 94%
Similar papers in this journal
Similar papers in this journal
- Substrate elasticity does not impact on DNA methylation changes during differentiation of pluripotent stem cells 95%
- Identification And Characterization Of A Large Source Of Primary Mesenchymal Stem Cells Tightly Adhered To Bone Surfaces Of Human Vertebral Body Marrow Cavities 92%
- Single-cell microencapsulation improves lung retention of endothelial colony forming cells after intravascular delivery and unmasks therapeutic benefit in severe pulmonary arterial hypertension 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.