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Design of a sequestration-based network with tunable pulsing dynamics

Nakamura, E.; Cuba Samaniego, C.; Blanchini, F.; Giordano, G.; Franco, E.

2024-03-24 synthetic biology
10.1101/2024.03.24.586474 bioRxiv
Show abstract

Incoherent feedforward networks exhibit the ability to generate temporal pulse behavior. However, exerting control over specific dynamic properties, such as amplitude and rise time, poses a challenge and is intricately tied to the networks implementation. In this study, we focus on analyzing sequestration-based networks capable of exhibiting pulse behavior. By employing time-scale separation in the fast sequestration regime, we approximate the temporal dynamics of these networks. This approach allows us to establish a mapping that elucidates the impact of varying the kinetic rates and pulse specifications, including amplitude and rise time. Furthermore, we introduce a positive feedback mechanism to regulate the amplitude of the pulsing response.

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