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High-throughput nano-flow proteomics using a dual-column electrospray source

Staes, A.; Boucher, K.; Dufour, S.; Maia, T. M.; Timmerman, E.; Van Haver, D.; Pauwels, J.; Demol, H.; Vandenbussche, J.; Impens, F.; Devos, S.

2024-02-13 biochemistry
10.1101/2024.02.13.580084 bioRxiv
Show abstract

With current trends in proteomics, especially regarding clinical and low input (to single cell) samples, it is increasingly important to both maximize the throughput of the analysis and maintain as much sensitivity as possible. The new generation of mass spectrometers (MS) are taking a huge leap in sensitivity, allowing to analyze samples with shorter liquid chromatography (LC) methods while digging as deep in the proteome. However, the throughput can be doubled by implementing a dual column nano-LC-MS configuration. For this purpose, we used a dual-column setup with a two-outlet electrospray source, and compared it to a classic dual-column setup with a single-outlet source.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.