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Peptidoform analysis of IP-MS data allows detection of differentially present bait proteoforms

Sdelci, S.; Sdelci, S.; Kourtis, S.; Cianferoni, D.; Serrano, L.

2024-01-26 bioinformatics
10.1101/2024.01.23.576810 bioRxiv
Show abstract

While it is recognised that protein functions are determined by their proteoform state, such as mutations and post-translational modifications, methods to determine their differential abundance between conditions are limited. Here, we present a novel workflow for classical immunoprecipitation coupled to mass spectrometry (IP-MS) data that focuses on identifying differential peptidoforms of the bait protein between conditions, providing additional information about protein function.

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