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Normalized level set model for segmentation of low-contrast objects in 2- and 3- dimensional images

Baig, M. M. J.; Wang, Y. L.; Chung, S. H.; Stepanyants, A.

2024-01-15 bioengineering
10.1101/2024.01.12.574651 bioRxiv
Show abstract

Analyses of biomedical images often rely on accurate segmentation of structures of interest. Traditional segmentation methods based on thresholding, watershed, fast marching, and level set perform well in high-contrast images containing structures of similar intensities. However, such methods can under-segment or miss entirely low-intensity objects on noisy backgrounds. Machine learning segmentation methods promise superior performance but require large training datasets of labeled images which are difficult to create, particularly in 3D. Here, we propose an algorithm based on the Local Binary Fitting (LBF) level set method, specifically designed to improve the segmentation of low-contrast structures.

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