An Antibiotic Degrading Engineered Living Material Platform to Combat Environmental Antibiotic Resistance
Ozkul, G.; Kehribar, E. S.; Seker, U. O. S.
Show abstract
Presence of antibiotics in natural water bodies is a growing problem regarding the occurrence of antibiotic resistance among various species. This is mainly caused due to excessive use of medical and veterinary antibiotics as well as the lack of effective treatment processes for eliminating residual antibiotic from wastewaters. In this study, we introduce a genetically engineered biomaterial as a solution for effective degradation of one of the dominantly found antibiotics in natural water bodies. Our biomaterial harnesses laccase type enzymes which are known to attack specific type of antibiotics, i.e. fluoroquinolone type synthetic antibiotics, and as a result degradation occurs. The engineered biomaterial is built using Escherichia coli biofilm protein CsgA as a scaffold, which is fused separately to two different laccase enzymes with the SpyTag - SpyCatcher peptide-protein duo. The final material is implemented as a part of a living material system. Our proposed system can be integrated as living antibiotic degrading materials to help the combat with antibiotic resistance.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Elucidating the regulation of glucose tolerance through the interaction between the reaction product and active site pocket residues of a β-glucosidase from Halothermothrix orenii 94%
- Selection of streptococcal glucan-binding protein C specific DNA aptamers to inhibit biofilm formation 94%
- Synthetic and plant-derived multivalent galactans as modulators of cancer-associated galectins-3 and -9 93%
Similar papers in this journal
- High-yield Production of Recombinant Platelet Factor 4 Protein by Harnessing and Honing the Gram-negative Bacterial Secretory Apparatus 95%
- Characterization of the Corynebacterium glutamicum dehydroshikimate dehydratase QsuB and its potential for microbial production of protocatechuic acid 95%
- OverFlap PCR - a reliable approach for generation of plasmid DNA libraries containing random sequences without template bias. 95%
Similar papers in this journal
- A clickable photosystem I, ferredoxin, and ferredoxin NADP+ reductase fusion system for light-driven NADPH regeneration 93%
- Characterization of a novel mesophilic CTP-dependent riboflavin kinase and rational engineering to create its thermostable homologs 92%
- Assessing Lanthanide-Dependent Methanol Dehydrogenase Activity: The Assay Matters 92%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.