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Label-free 3-D molecular imaging of living tissues using Raman Spectral Projection Tomography

Stepula, E.; Walther, A. R.; Mehrotra, D.; Jensen, M.; Yuan, M. H.; Pedersen, S. V.; Gentleman, E.; Albro, M. B.; Hedegaard, M. A. B.; Bergholt, M. S.

2023-11-29 bioengineering
10.1101/2023.09.23.559025 bioRxiv
Show abstract

The ability to image tissues in three-dimensions (3-D) with label-free molecular contrast at mesoscale would be a valuable capability in biology and biomedicine. Here, we introduce Raman spectral projection tomography (RSPT) for volumetric molecular imaging with sub-millimeter spatial resolution. We have developed a RSPT imaging instrument capable of providing 3-D molecular contrast in transparent and semi-transparent samples. A computational pipeline for multivariate reconstruction was established to extract label-free spatial molecular information from Raman projection data. We demonstrate imaging and visualization of phantoms of various complex shapes with label-free molecular contrast. Finally, we apply RSPT as a novel tool for imaging of molecular gradients and extracellular matrix heterogeneities in fixed and live tissue-engineered constructs and explanted native tissues. RSPT imaging opens new possibilities for label-free molecular monitoring of tissues.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.