A hybrid receptor binding protein enables phage F341 infection of Campylobacter by binding to flagella and lipooligosaccharides
Ostenfeld, L. J.; Sorensen, A. N.; Neve, H.; Vitt, A.; Klumpp, J.; Sorensen, M. C. H.
Show abstract
Flagellotropic bacteriophages are interesting candidates as therapeutics against pathogenic bacteria dependent on flagellar motility for colonization and causing disease. Yet, phage resistance other than loss of motility has been scarcely studied. Here we developed a soft agar assay to study flagellotropic phage F341 resistance in motile Campylobacter jejuni. We found that phage adsorption was prevented by diverse genetic mutations in the lipooligosaccharides forming the secondary receptor of phage F341. Genome sequencing showed phage F341 belongs to the Fletchervirus genus otherwise comprising capsular-dependent C. jejuni phages. Interestingly, phage F341 encodes a hybrid receptor binding protein (RBP) predicted as a short tail fiber showing partial similarity to RBP1 encoded by capsular-dependent Fletchervirus, but with a receptor binding domain similar to tail fiber protein H of C. jejuni CJIE1 prophages. Thus, C. jejuni prophages may represent a genetic pool from where lytic Fletchervirus phages can acquire new traits like recognition of new receptors.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- The Bacillus phage SPβ and its relatives: A temperate phage model system reveals new strains, species, prophage integration loci, conserved proteins and lysogeny management components 97%
- A lipopolysaccharide-dependent phage infects a pseudomonad phytopathogen and can evolve to evade phage resistance 96%
- Isolation and characterization of a phage collection against Pseudomonas putida 96%
Similar papers in this journal
Similar papers in this journal
- Closely related Vibrio alginolyticus strains encode an identical repertoire of prophages and filamentous phages 96%
- Four novel Caudoviricetes bacteriophages isolated from Baltic Sea water infect colonizers of Aurelia aurita 95%
- Genome sequencing of the bacteriophage CL31 and interaction with the host strain Corynebacterium glutamicum ATCC 13032 95%
Similar papers in this journal
- Four novel Picornaviruses viruses detected in Magellanic Penguins (Spheniscus magellanicus) in Chile 90%
- Discovery and characterisation of new phage targeting uropathogenic Escherichia coli 90%
- Plaque Size Tool: an automated plaque analysis tool for simplifying and standardising bacteriophage plaque morphology measurements 90%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.