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qMaLioffG: A single green fluorescent protein FLIM indicator enabling quantitative imaging of endogenous ATP

Arai, S.; Itoh, H.; Vu, C. Q.; Nakayama, M.; Oshima, M.; Morita, A.; Okamoto, K.; Okuda, S.; Teranishi, A.; Osawa, M.; Tamura, Y.; Nonoyama, S.; Takuma, M.; Fujie, T.; Sarker, S. R.; Sudhaharan, T.; Kiya, T.; Lane, E. B.; Kitaguchi, T.

2023-08-31 biophysics
10.1101/2023.08.29.555275 bioRxiv
Show abstract

The widespread use of fluorescence lifetime imaging microscopy (FLIM) for quantitative imaging is hindered by the limited availability of a FLIM-based genetically encoded indicator using a conventional 488 nm laser. Here, we present qMaLioffG, a single green fluorescent protein FLIM indicator showing a fluorescence lifetime change in ATP concentration within the physiological range. This allows quantitative imaging of endogenous ATP to investigate cellular energy status of different cell types.

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