Multi-species benchmark analysis for LC-MS/MS validation and performance evaluation in bottom-up proteomics
Jumel, T.; Shevchenko, A.
Show abstract
We present an instrument-independent benchmarking procedure and software (LFQ_bout) for validation and comparative evaluation of the performance of LC-MS/MS and data processing workflows in bottom-up proteomics. It enables back-to-back comparison of common and emerging workflows, e.g. diaPASEF or ScanningSWATH, and evaluates the impact of arbitrary, inadequately documented settings or black-box data processing algorithms. The procedure enhances the overall performance and quantitative accuracy while enabling the detection of major error types.
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