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Biocompatible hydrogel electrodeposition enables the simultaneous preparation of multi-microinterfaces for ligand bioconjugation and multiplexed electrochemical detection

Juska, V. B.; Moukri, N.; Estrela, P.; Maxwell, G. D.; Hendricks, S.; Santillan-Urquiza, E.; O'Brien, B.; Patella, B.; Inguanta, R.; O'riordan, A.

2023-08-18 bioengineering
10.1101/2023.08.17.553669 bioRxiv
Show abstract

Affinity based electrochemical biosensing systems with integrated miniaturised interfaces has enabled key advancement toward rapid, sensitive, precise and deployable detection platforms. Manufacturing silicon micro/nano devices for biology interface has been a highly promising platform to design and develop electrochemical sensors for the detection of very low concentrations of the target molecules. However, the biofouling challenge of the biosensors when the surface is exposed to a complex matrix such as blood, serum, milk, has been a road blocker. Here we introduce a simple, rapid formation of an anti-biofouling coating onto several electroactive surface areas present on a single chip simultaneously. Using such a multiplexed surface, we were able to investigate the optimum working conditions on-chip. Concentrating on two individual bioassay platforms for stress biomarkers, haptoglobin and cortisol, we demonstrate the broad applicability of the developed universal platform with excellent performance in bovine serum and correlation with conventional ELISA using milk samples.

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