A fluorescent sex-sorting technique for insects with the demonstration in Drosophila melanogaster
Liu, J.; Rayes, D.; Akbari, O.
Show abstract
Recent advances in insect genetic engineering offer alternative genetic biocontrol solutions to control populations of pests and disease vectors. While success has been achieved, sex-sorting remains problematic for scaling many genetic biocontrol interventions. Here we describe the development of a sex-sorting technique for female and male selection with a proof-of-concept in D. melanogaster termed SEPARATOR (Sexing Element Produced by Alternative RNA-splicing of A Transgenic Observable Reporter). This approach utilizes dominant fluorescent proteins and differentially spliced introns to ensure sex-specific expression. The system has the potential for adaptability to various insect species and application for high-throughput insect sex-sorting.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Spatial control of gene expression in flies using bacterially derived binary transactivation systems. 94%
- Germline mutagenesis of Nasonia vitripennis through ovarian delivery of CRISPR-Cas9 ribonucleoprotein 94%
- A Sex specific homologue of snake Waprin is essential for Embryonic Development in the Red Flour Beetle, Tribolium castaneum 93%
Similar papers in this journal
- Development of an eco-friendly RNAi yeast attractive targeted sugar bait that silences the Shaker gene in spotted-wing drosophila, Drosophila suzukii 94%
- Development of efficient RNAi methods in the corn leafhopper Dalbulus maidis, a promising application for pest control 94%
- Engineered expression of the invertebrate-specific scorpion toxin AaHIT reduces adult longevity and female fecundity in the diamondback moth Plutella xylostella 93%
Similar papers in this journal
- Pigmentation loci as markers for genome editing in the Chagas disease vector Rhodnius prolixus 94%
- An OpIE2-DsRed marker disrupts female blood-feeding and shortens lifespan in the malaria vector Anopheles gambiae. 93%
- Marker-assisted mapping enables effective forward genetic analysis in the arboviral vector Aedes aegypti, a species with vast recombination deserts 93%
Similar papers in this journal
- Transgenic Drosophila lines for LexA-dependent gene and growth regulation 95%
- A forward genetic approach to mapping a P-element second site mutation identifies DCP2 as a novel tumor suppressor in Drosophila melanogaster 94%
- Transgenic tools for targeted chromosome rearrangements allow construction of balancer chromosomes in non-melanogaster Drosophila species 93%
Similar papers in this journal
- High-Throughput Tracking of Freely Moving Drosophila Reveals Variations in Aggression and Courtship Behaviors 93%
- An optimized protocol for assay for transposase-accessible chromatin by sequencing (ATAC-seq) library preparation from adult Drosophila melanogaster neurons 93%
- Actin bundles play different role in shaping scale as compare to bristle in mosquito Aedes aegypti. 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.