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Transcriptome mining of RNA viruses (family Totiviridae) in Eimeria necatrix and Eimeria stiedai

Nibert, M. L.; Xie, Y.; Xiao, J.; Gao, Y.; Liu, D.; Yang, G.; Tao, J.

2023-05-20 microbiology
10.1101/2023.05.20.541574 bioRxiv
Show abstract

Coccidian protozoa from the genus Eimeria are widespread parasites of vertebrates, causing serious disease (coccidiosis) and economic loss most notably in poultry. Several species of Eimeria are themselves infected with small RNA viruses assigned to the family Totiviridae. In this study, the sequences of two such viruses were newly determined, one of which represents the first complete protein-coding sequence of a virus from E. necatrix, an important pathogen of chickens, and the other of which is from E. stiedai, an important pathogen of rabbits. Sequence features of the newly identified viruses, compared with those of ones reported previously, provide several insights. Phylogenetic analyses suggest that these eimerian viruses constitute a well-demarcated clade, probably deserving of recognition as a distinct genus.

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