Development and Validation of a Multiplex Real-Time PCR Assay for Detection and Quantification of Streptococcus pneumoniae in Pediatric Respiratory Samples

2023-05-05 infectious diseases Title + abstract only

Show abstract

Streptococcus pneumoniae (Spn) is a bacterial pathogen that causes a range of disease manifestations in children, from acute otitis media to pneumonia, septicemia, and meningitis. Primary Spn laboratory diagnostic identification methods include culture, antigen testing, single-plex real-time PCR, and syndromic PCR panels. However, each method lacks sensitivity, specificity, and/or cost efficiency. We developed and validated a quantitative, multiplex PCR assay that uses three Spn genomic targets ...

Development and Validation of a Multiplex Real-Time PCR Assay for Detection and Quantification of Streptococcus pneumoniae in Pediatric Respiratory Samples

Predicted journal destinations