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Two artificial tears outbreak-associated cases of XDR Pseudomonas aeruginosa detected through whole genome sequencing-based surveillance

Sundermann, A. J.; Rangachar Srinivasa, V.; Mills, E. G.; Griffith, M. P.; Waggle, K. D.; Ayres, A. M.; Pless, L.; Snyder, G. M.; Harrison, L. H.; Van Tyne, D.

2023-04-17 infectious diseases
10.1101/2023.04.11.23288417 medRxiv
Show abstract

We describe two cases of XDR Pseudomonas aeruginosa infection caused by a strain of public health concern recently associated with a nationwide outbreak of contaminated artificial tears. Both cases were detected through database review of genomes in the Enhanced Detection System for Hospital-Associated Transmission (EDS-HAT), a routine genome sequencing-based surveillance program. We generated a high-quality reference genome for the outbreak strain from one of the case isolates from our center and examined the mobile elements encoding blaVIM-80 and blaGES-9 carbapenemases. We then used publicly available P. aeruginosa genomes to explore the genetic relatedness and antimicrobial resistance genes of the outbreak strain.

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