Using double cut in vitro assembled CRISPR/Cas9 to modify the genome of Coccidioides posadasii
Mead, H.; Teixeira, M. M.; Blackmon, A.; Kollath, D. R.; Barker, B. M.; Morales, M. M.; Bryant, M.; Itogawa, A.
Show abstract
Although the genus Coccidioides is divided into two closely related and putatively allopatric species, analysis shows that hybridization has occurred between species and at least one C. posadasii conserved fragment has introgressed into several C. immitis genomes in a population-specific manner. Transcript abundance in vitro and in vivo for ten ORFs in this introgressed region were measured for several isolates. We used signals of introgression and high mRNA transcript levels in the spherule as indicators of selection for genes related to critical biological processes involved in Coccidioides pathogenesis. The only transcript in the introgression region with significant expression was a gene that encodes for a betadefensin-like (DEFBL) peptide rich in serines and cysteines. Few virulence factors have been identified in Coccidioides, and we employed the CRISPR-Cas9 mediated gene deletion tool to delete this gene in Coccidioides.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Multi-locus phylogenetic network analysis of Ampelomyces mycoparasites isolated from diverse powdery mildews in Australia and the generation of two de novo genome assemblies 95%
- Trafficking of the Telomerase RNA using a Novel Genetic Approach 94%
- Investigation of the quorum-sensing regulon of the biocontrol bacterium Pseudomonas chlororaphis strain PA23 94%
Similar papers in this journal
- Csp1, A Cold-Shock Protein Homolog in Xylella fastidiosa Influences Pili Formation, Stress Response, and Gene Expression 95%
- Transformation of Gardnerella vaginalis with a Bifidobacterium-Escherichia coli shuttle vector plasmid 94%
- Expression of the Bacillus thuringiensis vip3A insecticidal toxin gene is activated at the onset of stationary phase by VipR, an autoregulated transcription factor 94%
Similar papers in this journal
- Simple Growth Conditions Improve Targeted Gene Deletion in Cryptococcus neoformans 96%
- Functional characterization of clinical isolates of the opportunistic fungal pathogen Aspergillus nidulans 96%
- A conserved machinery underlies the synthesis of a chitosan layer in the Candida chlamydospore cell wall 95%
Similar papers in this journal
- Establishment of an antimetabolite-based transformation system for the wood-decaying basidiomycete Phanerochaete chrysosporium 95%
- LaeA controls citric acid production through regulation of a citrate exporter encoding cexA in Aspergillus luchuensis mut. kawachii 94%
- Pan-azole- and multi-fungicide-resistant Aspergillus fumigatus is widespread in the United States 94%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.