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metaGE: Investigating Genotype-by-Environment interactions through meta-analysis

De Walsche, A.; Vergne, A.; Rincent, R.; Roux, F.; Nicolas, S. D.; Welcker, C.; Mezmouk, S.; Charcosset, A.; Mary-Huard, T.

2023-03-02 plant biology
10.1101/2023.03.01.530237 bioRxiv
Show abstract

Dissecting the genetic components of Genotype-by-Environment interactions is of key importance in the context of increasing instability and plant competition due to climate change and phytosanitary treatment limitations. It is widely addressed in plants using Multi-Environment Trials (MET), in which statistical modelling for genome-wide association studies (GWAS) is promising but significantly more complex than for single-environment studies. In this context, we introduce metaGE, a flexible and computationally efficient meta-analysis approach for the joint analysis of any MET GWAS experiment. To cope with the specific requirements of the MET context, metaGE accounts for both the heterogeneity of QTL effects across environments and the correlation between GWAS summary statistics acquired on the same or related set(s) of genotypes. Compared to previous GWAS in 3 plant species and a multi-parent population, metaGE identified known and new QTLs. It provided valuable insight into the genetic architecture of several complex traits and the variation of QTL effects conditional to environmental conditions.

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