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A First Ultrastructural and Immuno-Labelling Investigation of Western Red Cedar Leaves

Gowen, B. E.; Noshad, D.

2023-01-30 plant biology
10.1101/2023.01.28.526058 bioRxiv
Show abstract

A first morphological analysis of the leaves of Thuja plicata (Western Red Cedar, WRC), a commercially valuable tree species, is presented. The ultrastructural and immuno-labelling analyses were only possible after applying modifications to standard transmission electron microscopy (TEM) preparatory methodology to fully embed cedar leaves. We demonstrate an application of the technique after leaf exposure to the fungi Didymascella thujina (cedar leaf blight, CLB). The leaves resulting defence response was visualized to two plant defence proteins, {beta}-1,3 Glucanase and Chitinase. The TEM methodology modifications presented may generally apply to other plant species resistant to morphological analyses due to their thick leaf cuticles. The technique requires no more equipment than found in any basic Transmission Electron Microscopy Facility.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.