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Heterologous production of cyanophycin with Tatumella morbirosei cyanophycin synthetase

Swain, K.; Sharon, I.; Blackson, W.; Tekel, S.; Schmeing, T. M.; Nielsen, D. R.; Nannenga, B. L.

2023-01-10 molecular biology
10.1101/2023.01.09.523336 bioRxiv
Show abstract

Microbial production of biopolymers represents a promising, sustainable alternative to current approaches for plastic production. Cyanophycin synthetase 1 (CphA1) produces cyanophycin - an attractive biopolymer consisting of a poly-L-aspartic acid backbone decorated with L-arginine side groups. In this work, a series of CphA1 enzymes from different bacteria were screened for heterologous cyanophycin production in engineered Escherichia coli, from which it was found that CphA1 from Tatumella morbirosei (TmCphA1) was especially productive. TmCphA1 was capable of supporting up to ~2-fold greater yields of insoluble cyanophycin than any other tested CphA1 enzymes, including 10.8-times more than CphA1 from Synechocystis sp. PCC6308. Finally, using a bench-scale bioreactor, cyanophycin production by TmCphA1-expressing E. coli reached up to 1.9 g per liter of culture by 48 h.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.