Demonstration of in vivo engineered tandem duplications of varying sizes using CRISPR and recombinases in Drosophila melanogaster
Loehlin, D. W.; McClain, G. L.; Xu, M.; Kedia, R.; Root, E.
Show abstract
Tandem gene duplicates are important parts of eukaryotic genome structure, yet the phenotypic effects of new tandem duplications are not well-understood, in part owing to a lack of techniques to build and modify them. We introduce a method, Recombinase-Mediated Tandem Duplication (RMTD), to engineer specific tandem duplications in vivo using CRISPR and recombinases. We describe construction of four different tandem duplications of the Alcohol Dehydrogenase (Adh) gene in Drosophila melanogaster, with duplicated block sizes ranging from 4.2 kb to 20.7 kb. Flies with the Adh duplications show elevated ADH enzyme activity over unduplicated single copies. This approach to engineering duplications is combinatoric, opening the door to systematic study of the relationship between the structure of tandem duplications and their effects on expression.
Matching journals
The top 2 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Transgenic tools for targeted chromosome rearrangements allow construction of balancer chromosomes in non-melanogaster Drosophila species 97%
- A method for rapid selection of randomly induced mutations in a gene of interest using CRISPR/Cas9 mediated activation of gene expression. 96%
- Rapid self-selecting and clone free integration of transgenes into engineered CRISPR safe harbor locations in Caenorhabditis elegans 96%
Similar papers in this journal
- Testing a candidate meiotic drive locus identified by pool sequencing 95%
- Genomics of Natural Populations: Gene Conversion Events Reveal Selected Genes within the Inversions of Drosophila pseudoobscura 94%
- Loss of altruism in the social amoeba Dictyostelium discoideum is associated with the G protein-coupled receptor grlG 94%
Similar papers in this journal
- phiC31 integrase for recombination mediated single copy insertion and genome manipulation in C. elegans 96%
- Expanding the Caenorhabditis elegans auxin-inducible degron system toolkit with internal expression and degradation controls and improved modular constructs for CRISPR/Cas9-mediated genome editing 95%
- Site-specific recombination between inverted target sites generates dicentric/acentric chromosomes. 95%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.