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Viable Monkeypox virus in the environment of a patient room

Marimuthu, K.; Wong, J.; Lim, P. L.; Octavia, S.; Huan, X.; Ng, Y. K.; Yang, J. J.; Sutjipto, S.; Linn, K. Z.; Setoh, Y. X.; Ong, C. H. C.; Griffiths, J.; Farhanah, S.; Cheok, T. S.; Sulaiman, N. A. B.; Congcong, S. B.; Neves, E. S.; Loo, L. H.; Hakim, L.; Sim, S.; Lim, M.; Nazeem, M.; Vasoo, S.; Tham, K. W.; Ng, O. T.; Ng, L. C.

2022-09-17 infectious diseases
10.1101/2022.09.15.22280012 medRxiv
Show abstract

We conducted a prospective environmental surveillance study to investigate the air, surface, dust and water contamination of a room occupied by a patient infected with Monkeypox virus (MPXV) at various stages of his illness. The patient tested positive for MPXV from a throat swab and skin lesions. Environmental sampling was conducted in a negative pressure room with 12 unidirectional HEPA air changes per hour and daily cleaning of the surfaces. A total of 179 environmental samples were collected on days 7, 8, 13, and 21 of his illness. Air, surface, and dust contamination was highest during the first eight days of the illness, with a gradual decline to the lowest contamination level by day 21. Viable MPXV was isolated from surfaces and dust samples and no viable virus was isolated from the air and water samples. SynopsisInadequate research prevail on the mode of transmission for monkeypox virus. This study reports the findings of viable monkeypox virus from the patient environment, highlighting its implications for human health and impact on infection and prevention control measures.

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