Monitoring correlates of SARS-CoV-2 infection in cell culture using two-photon microscopy and a novel fluorescent calcium-sensitive dye
Domokos, M.; Szalay, G.; Cseri, L.; Kis, Z.; Palyi, B.; Foldes, G.; Kovacs, N.; Fulop, A.; Szepesi, A.; Hajdrik, P.; Csomos, A.; Zsembery, A.; Kadar, K.; Katona, G.; Mucsi, Z.; Rozsa, B. J.; Kovacs, E.
Show abstract
The organism-wide effects of viral infection SARS-CoV-2 are well studied, but little is known about the dynamics of how the infection spreads in time among or within cells due to the scarcity of suitable high-resolution experimental systems. Two-photon (2P) imaging combined with a proper subcellular staining technique has been an effective tool for studying mechanisms at such resolutions and organelle levels. Herein, we report the development of a novel calcium sensor molecule along with a 2P-technique for identifying imaging patterns associated with cellular correlates of infection damage within the cells. The method works as a cell viability assay and also provides valuable information on how the calcium level and intracellular distribution are perturbed by the virus. Moreover, it allows the quantitative analysis of infection dynamics. This novel approach facilitates the study of the infection progression and the quantification of the effects caused by viral variants and viral load.
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