Viral infection impacts the 3D subcellular structure of the abundant marine diatom Guinardia delicatula
Walde, M.; Camplong, C.; de Vargas, C.; Baudoux, A.-C.; Simon, N.
Show abstract
Viruses are key players in marine ecosystems where they infect abundant marine microbes. RNA viruses are emerging as key members of the marine virosphere. They have recently been identified as a potential source of mortality in diatoms, a group of microalgae that accounts for roughly 40% of the primary production in the ocean. Despite their likely importance, their impacts on host populations and ecosystems remain difficult to assess. In this study, we introduce an innovative tool approach that combines automated 3D confocal microscopy with quantitative image analysis and physiological measurements to expand our understanding of viral infection. We followed different stages of infection of the bloom-forming diatom Guinardia delicatula by the RNA virus GdelRNAV-04 until the complete lysis of the host. From 20h after infection, we observed quantifiable changes in subcellular host morphology and biomass. Our microscopy monitoring also showed that viral infection of G. delicatula induced the formation of auxospores as a probable defense strategy against viruses. Our method enables the detection of discriminative morphological features on the subcellular scale and at high throughput for comparing populations, making it a promising approach for the detection quantification of viral infections in the field in the future.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- High-temporal resolution of microbial food web dynamics and structure during phytoplankton blooms in the Baltic Sea 94%
- Seasonal dynamics of a glycan-degrading flavobacterial genus in a tidally-mixed coastal temperate habitat 93%
- Short-term changes in polysaccharide utilization mechanisms of marine bacterioplankton during a spring phytoplankton bloom 93%
Similar papers in this journal
Similar papers in this journal
- Life Cycle and Morphogenetic Differentiation in Heteromorphic Cell Types of a Cosmopolitan Marine Microalga 95%
- Mapping the transcriptional landscape of algal resistance to viral infection reveals a core expression program 94%
- Phagocytosis underpins the biotrophic lifestyle of intracellular parasites in the class Phytomyxea (Rhizaria) 94%
Similar papers in this journal
- Extracellular vesicles from saxitoxin-producing strains of the cyanobacterium Raphidiopsis raciborskii and investigation of their potential allelopathic effect 93%
- Compartmentalization of mRNAs in the giant, unicellular green algae Acetabularia acetabulum 93%
- SAMMBA is a high-throughput pipeline for isolating and phenotyping macroalgal strains 92%
Similar papers in this journal
- Prochlorococcus rely on microbial interactions rather than on chlorotic resting stages to survive long-term nutrient starvation 90%
- Massive accumulation of strontium and barium in diplonemid protists 90%
- Generation of comprehensive ecosystems-specific reference databases with species-level resolution by high-throughput full-length 16S rRNA gene sequencing and automated taxonomy assignment (AutoTax) 90%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.