Application of RT-LAMP-CRISPR-Cas13a technology to the detection of OXA-48 producing Klebsiella pneumoniae
Ortiz-Cartagena, C.; Blasco, L.; Fernandez-Garcia, L.; Pacios, O.; Bleriot, I.; Lopez Diaz, M.; Fernandez-Cuenca, F.; Canton, R.; Tomas, M.
Show abstract
Carbapenem-resistant pathogens have been recognized as a health concern because of their ability to cause severe infections and because they are difficult to detect in laboratories. Researchers are making great efforts to develop a diagnostic technique with high levels of sensitivity and specificity, as accurate, early diagnosis is required to prevent the spread of these microorganisms and improve the prognosis of patients. In this context, CRISPR-Cas systems are proposed as promising tools for the development of diagnostic techniques due to their high specificity: Cas13 endonuclease discriminates single nucleotide changes and displays collateral activity against single-stranded RNA molecules. This technology is usually combined with isothermal pre-amplification reactions in order to increase the sensitivity of diagnosis. We have developed an RT-LAMP-CRISPR-Cas13a-based assay for the detection of Klebsiella pneumoniae OXA-48 producer strains in clinical samples without the need for RNA extraction. The assay exhibited 100 % specificity, sensitivity, positive predictive value and negative predictive value.
Matching journals
The top 8 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Transmissible antimicrobial resistance in Escherichia coli isolated from household drinking water in Ibadan, Nigeria 96%
- Occurrence, identification and antibiogram signatures of selected Enterobacteriaceae from Tsomo and Tyhume rivers in the Eastern Cape Province, Republic of South Africa. 95%
- A Novel Dual Probe-based Method for Mutation Detection using Isothermal Amplification 95%
Similar papers in this journal
- RT-LAMP-CRISPR-Cas13a technology as a promising diagnostic tool for the SARS-CoV-2 virus 96%
- Direct colorimetry of imipenem decomposition as a novel cost effective method for detecting carabapenamase producing bacteria 96%
- Acinetobacter baumannii and cefiderocol between cidality and adaptability 95%
Similar papers in this journal
- On-site genomic epidemiological analysis of antimicrobial-resistant bacteria in Cambodia with portable laboratory equipment 95%
- Correlation of pathogenic factors with antimicrobial resistance of clinical Proteus mirabilis strains 95%
- The LAMP-CRISPR-Cas13a technique for detecting CBASS-mechanism of phage resistance in bacteria 95%
Similar papers in this journal
- A Novel Air-Dried Multiplex High Resolution Melt Assay For The Detection Of Extended Spectrum Beta-Lactamase And Carbapenemase Genes 96%
- Genomic characterisation of an mcr-1 and mcr-3-producing Escherichia coli strain isolated from pigs in France 96%
- A novel plasmid-mediated PAC β-lactamase (PAC-2) variant confers resistance to ceftazidime-avibactam in Klebsiella quasipneumoniae ST526, in Colombia. 96%
Similar papers in this journal
- A novel platform to accelerate antimicrobial susceptibility testing in Neisseria gonorrhoeae using RNA signatures 95%
- resaCPE: A Rapid, Low-Cost Colourimetric Assay for the Detection of Carbapenemase-Producing Enterobacterales 95%
- A multicenter evaluation of a novel microfluidic rapid AST assay for Gram-negative bloodstream infections 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.