Exploring DTI-derived metrics to non-invasively track recellularisation in vascular tissue engineering
Tornifoglio, B.; Stone, A. J.; Mathieu, P. S.; Fitzpatrick, E.; Kerskens, C.; Lally, C.
Show abstract
Despite significant growth in the field of tissue engineering over the past decades, non-invasive, non-destructive methods to characterise recellularisation of grafts are lacking. Here, we investigate a non-invasive magnetic resonance imaging technique, diffusion tensor imaging (DTI), within acellular and recellularised vascular grafts. Using two decellularised porcine carotid grafts, smooth muscle cells were cultured dynamically for two weeks with terminal time points at day 3, 7, and 14. Grafts were fixed at each time point and investigated by DTI in an ex vivo set up. Semi-quantitative histology was carried out to investigate collagen, elastin, and cell density changes over time. DTI-derived metrics, namely the fractional anisotropy, mean diffusivity and tractography, not only were significantly different between day 3 and day 7 grafts, but also distinguished between acellular and recellularised grafts. Specifically, within the wet decellularised grafts, increasing fractional anisotropy was strongly correlated to increasing cell density. The results from this study show, for the first time, DTIs place in the field of tissue engineering, offering non-invasive, non-destructive insight into graft recellularisation.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Inflammatory and regenerative processes in bioresorbable synthetic pulmonary valves up to 2 years in sheep: Spatiotemporal insights augmented by Raman microspectroscopy 94%
- Advanced Manufacturing of Coil-Reinforced Multilayer Vascular Grafts to Optimize Biomechanical Performance 93%
- Hierarchically Vascularized and Implantable Tissue Constructs created through Angiogenesis from Tissue-Engineered Vascular Grafts 92%
Similar papers in this journal
- Diffusion tensor imaging and arterial tissue: establishing the influence of arterial tissue microstructure on fractional anisotropy, mean diffusivity and tractography 97%
- 3D imaging and morphometry of the heart capillary system in spontaneously hypertensive rats and normotensive controls 93%
- Tissue-engineered collagenous fibrous cap models to systematically elucidate atherosclerotic plaque rupture. 93%
Similar papers in this journal
Similar papers in this journal
- In vitro evaluation of Escherichia coli and Staphylococcus aureus translocation in 3D printed material 92%
- Tyrosine-Derived Polycarbonate Nerve Guidance Tubes Elicit Pro-Regenerative Extracellular Matrix Deposition When Used to Bridge Segmental Nerve Defects in Swine 91%
- Label-free cleared tissue microscopy and machine learning for 3D histopathology of biomaterial implants 91%
Similar papers in this journal
- Endoluminal catheter pulsed field ablation for the treatment of atherosclerotic vascular disease 92%
- Biomechanical Characterization Of Porcine Lower Limb Arteries For Preclinical Evaluation Of Peripheral Vascular Devices 91%
- Impact of residual intimal flap displacement post-TEVAR on TBAD haemodynamics in compliant, patient-specific CFD simulations informed by MRI 90%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.