Development of genome-driven, lifestyle-informed primers for identification of the cereal infecting pathogens Xanthomonas translucens pathovars undulosa and translucens.
Roman-Reyna, V.; Curland, R. D.; Velez-Negron, Y.; Ledman, K. E.; Gutierrez, D.; Beutler, J.; Butchacas, J.; Brar, G. S.; Roberts, R.; Dill-Macky, R.; Jacobs, J. M.
Show abstract
Bacterial leaf streak, blight and black chaff caused by Xanthomonas translucens pathovars are major diseases affecting small grains. Xanthomonas translucens pv. translucens and X. translucens pv. undulosa are seedborne pathogens that cause similar symptoms on barley, but only X. translucens pv. undulosa causes bacterial leaf streak of wheat. Recent outbreaks of X. translucens have been a concern for wheat and barley growers in the Northern Great Plains and Upper Midwest; however, there are limited diagnostic tools for pathovar differentiation. We developed a multiplex PCR based on whole-genome differences to distinguish X. translucens pv. translucens and X. translucens pv. undulosa. We validated the primers across different Xanthomonas and non-Xanthomonas strains. To our knowledge, these are the first multiplex PCR to distinguish X. translucens pv. translucens and X. translucens pv. undulosa. These molecular tools will support disease management strategies enabling detection and pathovar incidence analysis of X. translucens.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Novel clonal lineages of wheat stem rust in the Southern Cone of South America 94%
- Identification of Halo Blight Disease on Oat in Idaho and Exploration of Resistant Sources in Oat, Barley and Wheat 94%
- New races with wider virulence indicate local evolution of Puccinia striiformis f. sp. tritici in South America 93%
Similar papers in this journal
- Genomic acquisitions in emerging populations of Xanthomonas vasicola pv. vasculorum infecting corn in the U.S. and Argentina 94%
- A Re-evaluation of phylogenomic data reveals that current understanding in wheat blast population biology and epidemiology is obfuscated by oversights in population sampling 92%
- Characterization of three resistance-breaking isolates of sugarcane mosaic virus from Rwanda and implications for maize lethal necrosis 92%
Similar papers in this journal
- A framework for gene mapping in wheat demonstrated using the Yr7 yellow rust resistance gene 93%
- Genome-wide association mapping identifies Yellow Rust resistance locus in Ethiopian Durum Wheat germplasm 92%
- Whole genome resequencing and comparative genome analysis of three Puccinia striiformis f. sp. tritici pathotypes prevalent in India 92%
Similar papers in this journal
- When time really is money: in situ quantification of the strobilurin resistance mutation G143A in the wheat pathogen Blumeria graminis f. sp. tritici 93%
- Bivariate analysis of barley scald resistance with relative maturity reveals a new major QTL on chromosome 3H 93%
- Development of a bioassay guided genome mining approach for antifungal natural product discovery from pseudomonads 92%
Similar papers in this journal
- A conserved fungal Knr4/Smi1 protein is vital for maintaining cell wall integrity and host plant pathogenesis 92%
- Avirulence depletion assay: combining R gene-mediated selection with bulk sequencing for rapid avirulence gene identification in wheat powdery mildew 92%
- Variability in an effector gene promoter of a necrotrophic fungal pathogen dictates epistasis and effector-triggered susceptibility in wheat 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.