Wide-Field Multicontrast Nonlinear Microscopy for Histopathology
Uribe Casta\~no, L.; Mirsanaye, K.; Golaraei, A.; Kontenis, L.; Done, S. J.; Stambolic, V.; Akens, M.; Wilson, B.; Barzda, V.
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A multicontrast polarimetric wide-field second harmonic generation (SHG) and multiphoton excitation fluorescence (MPF) microscope is optimized for large area imaging of hematoxylin and eosin (H&E) stained and unstained histology slides. The bleaching kinetics of MPF and SHG are examined with various laser intensities at different pulse repetition rates to determine the optimal wide-filed imaging conditions for H&E stained histology slides. Several polarimetric parameters are used to investigate the organization of extracellular matrix collagen in the histology samples.
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