Sub-cellular dynamic investigation of the multi-component drug on the gastric cancer cell BGC823 using Raman spectroscopy
shang, w.; ye, a.; fang, t.
Show abstract
The potential of Raman spectroscopy in anticancer drug study has been demonstrated, yet its ability to character systematic cellular changes caused by multi-component drugs has not been explored. Here we used micro-Raman spectroscopy combined with bright field imaging to study Compound Kushen injection (CKI) at a sub-cellular level including intracellular vesicles(IVs). In our report, CKI caused dysfunction of DNA replication and repair was displayed by Raman spectrum (RS) from the cell nucleus. Meanwhile, the dynamics of CKI induced intracellular vesicles and cell component deconstruction was delineated by RS from the cytoplasm and IVs. The lipids-related biomolecular changes were also presented by the cytoplasm RS: the lipids level in the cytoplasm first descended then uprising. In conclusion, this study validated the mechanism and displayed the dynamics of CKI in treating cancer cells. We proved the capability of subcellular micro-Raman spectroscopy for detecting systematic cellular changes and its application for multi-component drug evaluation.
Matching journals
The top 11 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- In situ Quantification of Biomolecular Concentration of Cytoplasmic Membraneless Organelles in a Living Cell 96%
- Following embryonic stem cells, their differentiated progeny, and cell-state changes during iPS reprogramming by Raman spectroscopy 94%
- Label-Free Quantification of Apoptosis and Necrosis Using Stimulated Raman Scattering Microscopy 93%
Similar papers in this journal
Similar papers in this journal
Similar papers in this journal
- Oxidative stress induced by tBHP in human normal colon cells by label free Raman spectroscopy and imaging.The protective role of natural antioxidants in the form of β-carotene 95%
- Mitoxantrone dihydrochloride, an FDA approved drug, binds with SARS-CoV-2 NSP1 C-terminal 92%
- Synergistic effects of conductivity and cell-imprinted topography of chitosan-polyaniline based scaffolds for neural differentiation of adipose-derived stem cells 92%
Similar papers in this journal
- Ultra-sensitive nanozyme-based chemiluminescence paper test for rapid diagnosis of SARS-CoV-2 infection 93%
- RaMALDI: enabling simultaneous Raman and MALDI imaging of the same tissue section 91%
- Synthetic Nanobody-Functionalized Nanoparticles for Accelerated Development of Rapid, Accessible Detection of Viral Antigens 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.