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Synthesis of cell-laden alginate microgels with tunable compositions based on microfluidic pico-injection technique

Zhang, Y.; Mao, A.; Mooney, D. J.; Weitz, D. A.

2022-01-11 bioengineering
10.1101/2022.01.09.475570 bioRxiv
Show abstract

We report a microfluidic pico-injection-based approach for reliably generating monodisperse cell-laden alginate microgels whose composition can be tuned in situ through modulation of the cross-linker concentration. Separating the gelation from emulsification allows for a better control over the microgel size with a microfluidic drop-maker, and an instant adjustment of the microgel composition with a pico-injector.

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